Product Detail

Anti-Factor IX

Clone GMA-001 (BC-2)

Epitope:  Factor IX gla domain

Immunogen:  Human factor IX

Host:  Murine

Clonality:  Monoclonal

Hybridoma:  NS-1 x murine spleen cells (Balb/c)

Isotype:  IgG2a

Specificity & Applications: 

Solid-phase ELISA:  Antibody binds human factor IX, gla domain.

 

Immunoblotting:  currently no blotting data

Product Form:   Lyophilized from a 1 mg/ml solution with 10 mM NaH2PO4, 0.15 M NaCl, 1.0% mannitol, pH 7.4.  Concentration determined by absorbance measurement at 280 nm and using an extinction coefficient of 1.4 (E0.1%).

Purity:  Confirmed >98% pure by SDS-PAGE

Working Concentration:  Approximately 1-5 μg/ml.  Researcher should titer antibody in specific assay.

Country of Origin:  USA

References: 

Jian-Ke Tie, Da-Yun Jin, David L. Straight, Darrel W. Stafford. Functional study of the vitamin K cycle in mammalian cells. Blood. 2011; 117: 2967-2974.

Feuerstein GZ, Toomey JR, Valocik R, Koster P, Patel A, Blackburn MN. An inhibitory anti-factor IX antibody effectively reduces thrombus formation in a rat model of venous thrombosis. Thromb Haemost. 1999; 82: 1443–1445.

Feuerstein GZ, Patel A, Toomey JR, Bugelski P, Nichols AJ, Church WR, Valocik R, Koster P, Baker A, Blackburn MN. Antithrombotic efficacy of a novel murine antihuman factor IX antibody in rats. Arterioscler Thromb Vasc Biol.1999; 19: 2554–2562.