Anti-Factor VIII
Clone GMA-012
Epitope: A2 domain residues 497-510 and 584-593, a discontinuous epitope1
Immunogen: Human factor VIII
Host: Murine
Clonality: Monoclonal
Hybridoma: NS-1 x murine spleen cells (Balb/c)
Isotype: IgG1
Specificity:
Solid-phase ELISA: Antibody binds the A2 domain of human factor VIII and human factor VIIIa.
Immunoblotting: Antibody binds human factor VIII, specifically the heavy chain, at an approximate molecular weight of 90-200 kD, under reduced conditions and 280 kD under non-reduced conditions.
Product Form: Lyophilized from a 1 mg/ml solution with 10 mM NaH2PO4, 0.15 M NaCl, 1.0% mannitol, pH 7.4. Concentration determined by absorbance measurement at 280 nm and using an extinction coefficient of 1.4 (E0.1%).
Purity: Confirmed >98% pure by SDS-PAGE
Working Concentration: Approximately 1-5 μg/ml. Researcher should titer antibody in specific assay.
Country of Origin: USA
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