Product Detail

Anti-Antithrombin

Clone GMA-800

Epitope:  Not determined.

Immunogen:   Human anti-thrombin III

Host:  Murine

Clonality:  Monoclonal

Hybridoma:  NS-1 x murine spleen cells (Balb/c)

Isotype:  IgG1

Specificity & Applications: 

Solid-phase ELISA:    Antibody binds human antithrombin (ATIII) in solid-phase ELISA.  It binds human and baboon ATIII in plasma, but not ATIII in bovine, dog or rabbit plasma. 

 

Immunoblotting:  Antibody binds human anti-thrombin, approximate molecular weight of 58 kD, under reduced and non-reduced conditions.

 

Other Information: The dissociation constant of this antibody is 2.0 x 10-9 M/L (BIACORE).  Antibody inhibits plasma ATIII activity in chromogenic thrombin assays in the presence of excess heparin and blocks purified IIa inhibition activity and purified Xa inhibition activity in the absence of heparin

 

 Product Form:   Lyophilized from a 1 mg/ml solution with 10 mM NaH2PO4, 0.15 M NaCl, 1.0% mannitol, pH 7.4.  Concentration determined by absorbance measurement at 280 nm and using an extinction coefficient of 1.4 (E0.1%).

Purity:  Confirmed >98% pure by SDS-PAGE

Working Concentration:  Approximately 1-5 μg/ml.  Researcher should titer antibody in specific assay.

Country of Origin:  USA