Quasi-Elastic Light Scattering

Aggregation Analysis by Quasi-Elastic Light Scattering  

 

Immunoglobulins can form dimers, trimers and larger aggregates during antibody purification, freeze/thaw, lyophilization and processes such as buffer exchange.  Aggregation can lead to a loss of yield and decreased antibody activity.  If aggregated antibody is used for in-Vivo experiments the aggregates can deposit in tissues and generate inflammatory and/or immune responses. 

 

Quasi-Elastic Light Scattering (QELS) also known as Dynamic Light Scattering (DLS) is a method for analysis of particle size in a solution.  Utilizing the Zetasizer Nano by Malvern, antibody solutions can be analysed quickly for the presence of aggregates.  QELS along with Size Exclusion Chromatography (SEC) provides important imformation on the physical properties of an antibody in solution.

	detects size range of 0.6 nm to 6 um
	Low concentration and sample volume requirements
	Noninvasive, measures a large population of particles, similar results to analytical ultracentrifugation

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